RFID technology can be applied to frozen sample storage

An RFID-based system to track frozen human tissues and cells, as well as harmful substances, can reduce the need for laboratories to handle these items in the laboratory. The system, called FreezerPro, is provided by RURO and designed specifically for the frozen sample storage market, so that users can know which samples are stored in the refrigerator, which samples have been moved, and can be easily found. The sample is usually placed in a clean plastic tube and placed in a storage container. However, in large-capacity laboratories, samples are also placed in plastic trays with recesses. The trays are about 2.5 inches wide and 4 inches long and are placed one after the other in a storage box with nearly 400 trays. Whether using vials or trays, cold storage may contain thousands of samples.

Labs using the RURO system store samples in vials. By using this system, laboratories can have better safety when managing samples, including high-risk products such as frozen bacteria or viruses, and valuable items such as stem cell research substances. They can also use this system to quickly complete the storage audit.

Historically, refrigerated laboratories have faced the challenge of tracking samples in bottles in storage boxes in cold stores. Samples are stored between minus 90 degrees Celsius and minus 190 degrees Celsius. Most vials and trays have either a serial number that can be identified or have pen-written descriptions or barcode labels around the vial or tray. The sample container stored in the box contains approximately 64 to 100 samples. In order to ensure that a particular sample is in a specific location and the sample is not mistaken, laboratory technicians must maintain a close record of the location of the sample container. Whenever a vial is removed, its serial number must be recorded, or the barcode label must be scanned to ensure that the appropriate sample has been removed. In both cases, the frost on the sample container makes this work difficult. To identify a vial, it is often necessary to manually remove the frost, explains Doug Miliken, Director of Sales at RURO. Since samples stored in vials can be hazardous, excessive handling of such samples can be very problematic.

With the FreezerPro system, labs can use desktop readers and RFID tags to track samples. On each container's FreezerPro label, the lab prints a bar code serial number for use as needed, as well as readable textual information about the sample, and is programmed in the EPC Gen2 Passive UHF tag. A unique ID number. Samples are stored in boxes with RFID tags. The FreezerPro software connects the ID number of each box label with the ID number of the sample container label. As new vials or trays are manufactured, the lab codes and prints labels on Zebra Technologies' label printers and attaches them to the sample container. Once it is labeled and the sample is placed in the box, simply place the box on the reader and the software can then get the ID number of all the samples, saving the staff the time to identify the samples one by one.

One of the laboratories in both laboratories started using the system a year ago and is currently tracking about 30,000 human biological samples. The laboratory selected an RFID system to reduce the amount of work required to verify the correct use of samples in research work and reduce the time it takes to inventory the cold storage. The laboratory must keep track of these samples during the audit and the audit will be conducted at least once a year.

With up to 50 researchers in the lab, tracking each vial to ensure their safety was the biggest challenge the lab faced. With the new system, researchers simply log into the FreezerPro software on the lab's back-end system and type in the name of the specific sample they want to use for their job. According to Milliken, the software will show which refrigerators and which boxes they are looking for.

The lab has three Impinj Speedway desktop RFID readers, of which two are installed in the cold storage area and the other is installed in the lab. When a sample is taken from the refrigerator, the technician needs to place the entire storage box on the reader. The reader obtains the ID number of the box label and the ID number of all these samples in the box, and then sends the information to FreezerPro software. The software compiles these ID numbers and displays the results on the computer monitor, including any one of the samples is lost or dropped. Wrong warning.

The software displays the graphics inside the box. Each storage space in the box displays a colored logo on the graph, indicating the type of sample in the container for each storage space. It also determines whether the sample the researcher is looking for is in a box. In this way, it is not necessary to open each box when looking for vials. When the box is returned to the refrigerator, the box is again placed on the desktop reader. The reader reads the ID number of the box and the ID number of the bottle's RFID tag and sends it to the FreezerPro software, indicating that the item has been returned to the refrigerator. .

The second laboratory was to store US military samples. The laboratory used a system similar to that of the first laboratory. The lab started using the system about five months ago. In this laboratory, safety is the highest priority, the system is in place, and the most important thing is to ensure that the samples are in the right place. As each box is scanned, researchers can see if all the vials are identified. With frequent inventory counts, staff can know which sample is missing and can reduce the time spent searching for samples. The lab uses two FreezerPro readers to track about 2000 samples.

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